Description
An Optimal MPS Workflow
The PowerSeq® 46GY System contains reagents to amplify autosomal and Y-STR loci as small amplicons (140–300bp) that can be used to prepare MPS libraries and generate sequencing data compatible with Illumina® TruSeq® and MiSeq® technologies. With this workflow, both sequence and allele length polymorphism in the autosomal and Y-STR loci can be identified, thereby increasing the statistical power of inclusion. MPS also eliminates the problem of overlapping alleles completely, allowing us to target more alleles in the multiplex.
The combination of these STR loci and Amelogenin makes this multiplex an effective tool for human identification using MPS, while maintaining compatibility with existing databases worldwide. A combination of familiar markers in the PowerSeq® 46GY System and data analysis software package GeneMarker®HTS from SoftGenetics, LLC, offers a smooth transition to include MPS into your workflow, ensuring that you get the data you want.
Learn more about MPS in this blog post .
New! This application note provides an alternate protocol for the PowerSeq® 46GY System for use of the KAPA HyperPrep Library Prep Kit for library preparation and ProNex® chemistry for bead purification.
