Promega, R6021, BamHI

Name: BamHI
Brand: Promega
SKU: R6021

Capable of digesting DNA in 15 minutes or less, Active and capable of digestion directly in GoTaq® Green Master Mix, Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications, Configuration: 10u/µl/2, 500u

Catalog Number: R6021

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Category

Restriction Enzymes

TagsCloning and DNA Markers Restriction Enzymes

Description

Cut Site:
G ▼ GATC C C CTAG ▲ G
Usage Notes:
Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn 2+ .
Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
Incubation Conditions: Buffer E. 37°C.
Source: Bacillus amyloliquefaciens H.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl 2 , 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
*Buffer A is not recommended due to potential star activity.
Frequency of Cutting:

Specifications

General

Catalog Number	R6021

Ordering

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