Promega, R6111, PstI

Name: PstI
Brand: Promega
SKU: R6111

Capable of digesting DNA in 15 minutes or less, Active and capable of digestion directly in GoTaq® Green Master Mix, Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications, Configuration: 10u/µl/3, 000u

Catalog Number: R6111

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Category

Restriction Enzymes

TagsCloning and DNA Markers Restriction Enzymes

Description

Cut Site:
C TGCA ▼ G G ▲ ACGT C
Notes:
Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
Ends generated by PstI can be directly ligated to ends generated by NsiI. Neither restriction site is regenerated in the ligation product.
Incubation Conditions: Buffer H. 37°C.
Source: Recombinant E. coli strain.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
Frequency of Cutting:

Specifications

{"type": "table", "rows": [{"name": "Buffer H 10X Buffer", "conditions": {"Part #": "R008A", "Size": "1 × 1ml", "Concentration": "", "Available Separately": ""}}, {"name": "Bovine Serum Albumin, Acetylated", "conditions": {"Part #": "R396D", "Size": "1 × 150μl", "Concentration": "10mg/ml", "Available Separately": "View Product"}}, {"name": "PstI", "conditions": {"Part #": "R611A", "Size": "1 × 3,000u", "Concentration": "", "Available Separately": ""}}, {"name": "MULTI-CORE™ 10X Buffer", "conditions": {"Part #": "R999A", "Size": "1 × 0.25ml", "Concentration": "", "Available Separately": "View Product"}}]}

Ordering

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