Promega, R6435, NotI

Name: NotI
Brand: Promega
SKU: R6435

Capable of digesting DNA in 15 minutes or less, Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications, Genome qualified to ensure optimal performance in genomic analysis applications, Configuration: 10u/µl/1, 000u

Catalog Number: R6435

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Category

Restriction Enzymes

TagsCloning and DNA Markers Restriction Enzymes

Description

Cut Site:
GC ▼ GGCC GC CG CCGG ▲ CG
Usage Note: Ends generated using NotI can be directly ligated to ends generated using BstZI or Eco52I. BstZI and Eco52I sites are regenerated in the ligation product. NotI sites may not be regenerated.
Incubation Conditions: Buffer D. 37°C.
Source: Nocardia otitidis-caviarum.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 0.1% Triton® X-100, 500mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
Frequency of Cutting:

Specifications

{"type": "table", "rows": [{"name": "Buffer D 10X Buffer", "conditions": {"Part #": "R004A", "Size": "1 × 1ml", "Concentration": "", "Available Separately": ""}}, {"name": "Bovine Serum Albumin, Acetylated", "conditions": {"Part #": "R396D", "Size": "1 × 150μl", "Concentration": "10mg/ml", "Available Separately": "View Product"}}, {"name": "NotI", "conditions": {"Part #": "R643E", "Size": "1 × 1,000u", "Concentration": "", "Available Separately": ""}}, {"name": "MULTI-CORE™ 10X Buffer", "conditions": {"Part #": "R999A", "Size": "1 × 0.25ml", "Concentration": "", "Available Separately": "View Product"}}]}

Ordering

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