Promega, R6651, BclI

Name: BclI
Brand: Promega
SKU: R6651

Genome qualified to ensure optimal performance in genomic analysis applications, Configuration: 10u/µl/1, 000u

Catalog Number: R6651

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Category

Restriction Enzymes

TagsCloning and DNA Markers Restriction Enzymes

Description

Cut Site:
T ▼ GATC A A CTAG ▲ T
Notes:
Sensitive to dam methylation.
For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22 , 3640–59.
Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.
Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)
Source: Bacillus caldolyticus.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
Frequency of Cutting:

Specifications

General

Catalog Number	R6651

Ordering

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