Promega, R7103, SgfI

Name: SgfI
Brand: Promega
SKU: R7103

Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications, Genome qualified to ensure optimal performance in genomic analysis applications, Configuration: 8–12u/µl/250u

Catalog Number: R7103

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Category

Restriction Enzymes

TagsCloning and DNA Markers Restriction Enzymes

Description

Cut Site:
GCG AT ▼ CGC CGC ▲ TA GCG
Notes:
Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
Sensitive to CpG methylation.
SgfI is inactivated by freezing at –70°C.
Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
Incubation Conditions: Buffer C. 37°C.
Source: Streptomyces fulvissimis .
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
Frequency of Cutting:

Specifications

General

Catalog Number	R7103

Ordering

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