Promega, S2001, Coelenterazines

Luciferases from Renilla , Aequorea and other marine organisms are commonly used as indicators or reporters for studying cellular phenomena in expression assays in eukaryotic cells. Renilla luciferase is often used as a reporter of transcription regulation, whereas apoaequorin is often used as a calcium indicator. Other uses of coelenterazines include chemiluminescent detection of Reactive Oxygen Species (ROS) in cells or tissues. We offer the following coelenterazine analogs:
Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin. Formula: C 26 H 21 N 3 O 3 . Formula Weight: 423.5. Form: Film.
Coelenterazine-h imparts a luminescent intensity with its aequorin complex that is reported to be 10–20 times higher than that of native coelenterazine, making this derivative a useful tool for measuring small changes in Ca 2+ concentrations. Formula: C 26 H 21 N 3 O 2 . Formula Weight: 407.5. Form: Film.
Learn more about custom options for this product at: www.promega.com/custom/
References
Inouye, S. et al. (1997) Biochem. and Biophys. Res. Comm. 233 , 349–53.
Blanpain, C. et al. (2001) J. Biol. Chem. 276 , 23795–804.
Shimomura, O. et al. (1989) Biochem. J. 261 , 913–20.