Description
The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction. The assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) when higher ATP concentration is required (up to 5mM). The ADP-Glo™ Max Assay produces a strong signal that positively correlates with enzyme activity and can be adapted to a multitude of plate formats.
The assay is performed in two steps: first, after the completion of the ADP-producing reaction, an equal volume of ADP-Glo™ Reagent is added to terminate the reaction and deplete the remaining ATP. Second, the ADP-Glo™ Max Detection Reagent is added to simultaneously convert ADP to ATP, and the latter is converted to light in a coupled reaction with luciferase/luciferin.
The ADP-Glo™ Max Assay has a high dynamic range and produces a strong signal at low ATP to ADP conversion, making it well suited for screening low-activity ATPases such as drug membrane transporters and heat shock proteins. The assay produces minimal false hits and Z´ values of greater than 0.7.
We offer a large selection of Kinase Enzyme Systems. Visit www.promega.com/kinase/ to see the collection.
High signal strength at low ATP conversion makes the assay well suited for low-activity ATPases/kinases.
Sensitive to low concentrations of ADP, requiring less enzyme than other assays.
Use with virtually with any ADP-producing enzyme—enables researchers to screen a wider range of enzymes using a single platform.
Can be used at ATP concentrations up to 5mM, important for enzymes with high Ka m values for ATP and for mode of action studies.
Accurately measures ADP levels at a wide range of starting ATP concentrations; users assured that activity measured truly reflects enzyme activity and produces accurate IC 50 values comparable to radioactivity-based assays.
The ADP-Glo™ Max Assays contain enough reagent to perform the indicated number of reactions in 384-well format using 5μl, 5μl and 10μl of an enzyme reaction, ADP-Glo™ Reagent and ADP-Glo™ Max Detection Reagent, respectively, per sample.
Principle of the ADP-Glo™ Max Assay. The assay is performed in two steps: 1) after the ATPase or kinase reaction, ADP-Glo™ Reagent is added to terminate the reaction and deplete the remaining ATP; and 2) the ADP-Glo™ Max Detection Reagent is added to convert ADP to ATP and allow the newly synthesized ATP to be measured using a luciferase/luciferin reaction. The light generated correlates to ADP present and ATPase activity.
