Promega, V7601, MTase-Glo™ Methyltransferase Assay

Name: MTase-Glo™ Methyltransferase Assay
Brand: Promega
SKU: V7601

Measure Methyltransferase Activity Using a Universal Bioluminescent Assay, Simple add-and-read format, Use with any methyltransferase, No need to modify substrates, Configuration: 400 assays

Catalog Number: V7601

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Category

Substrate Solutions

TagsEpigenetics Research Kits and Reagents Methylation Analysis

Description

Measure Methyltransferase Activity Using a Universal Bioluminescent Assay
The MTase-Glo™ Methyltransferase Assay is a novel, bioluminescence-based assay that can be used to monitor the activities of methyltransferases (MTases) and their modulation by small molecules in many applications, including high-throughput screening.
Monitors formation of the reaction product S-adenosyl homocysteine (SAH) and can detect changes in activity of a broad range of methyltransferases, including DNA, protein, RNA and small molecule methyltransferases.
Used successfully for all classes of protein methyltransferases (lysine and arginine) and with different types of substrates (peptides, large proteins and even nucleosomes) to determine the specificity of these enzymes and their substrate requirements.

Specifications

{"type": "table", "rows": [{"name": "SAM, 1mM", "conditions": {"Part #": "A120B", "Size": "1 × 200μl"}}, {"name": "SAH, 15µM", "conditions": {"Part #": "A233B", "Size": "1 × 300μl"}}, {"name": "MTase-Glo™ Reagent, 10X", "conditions": {"Part #": "V760A", "Size": "1 × 1ml"}}, {"name": "MTase-Glo™ Detection Solution", "conditions": {"Part #": "V761A", "Size": "1 × 10ml"}}]}

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