Promega, V7682, GTPase-Glo™ Assay

Name: GTPase-Glo™ Assay
Brand: Promega
SKU: V7682

Catalog Number: V7682

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Category

Recombinant Growth Factors

TagsCell Signaling GPCR Signaling Assays

Description

Monitor a Variety of GTPase-modulating Proteins (i.e., GEFS, gapS)
To mediate the process of shuttling between the active and inactive forms, GTPases need the help of two families of proteins: GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). GEFs replace the GDP bound to inactive GTPase with GTP, making the enzyme active and transducing a wide variety of cellular signals. GTPases then require the help of GAPs to hydrolyze GTP and switch off the signaling cascade.
Stimulation of GTPase Activity with GEF
In this example, Ran GTPase activity is stimulated by RCC1, a guanine nucleotide exchange factor (GEF), and detected with the GTPase-Glo™ Assay
Reactions containing 2µM of wildtype or mutant Ras (RasG12V) plus 1µM of NF1-333 (GAP protein) in GTPase/GAP Buffer.
Reactions containing 2µM of wildtype or mutant Ran (RanE70A) plus 1µM of RanGAP (GAP protein) in GTPase/GAP Buffer.

Specifications

General

Catalog Number	V7682

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