Promega, V7990, Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay

Name: Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay
Brand: Promega
SKU: V7990

Sensitive, Bioluminescent Detection of Demethylase Activity, Easy to use add-and-read assay format, Universal assay can be used with any succinate-producing enzyme, Optimized for screening applications; low false hits, Configuration: 1, 000 assays

Catalog Number: V7990

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Category

ELISA Substrates

Pack Size1 vial

TagsEpigenetics Research Kits and Reagents Methylation Analysis

Description

Simple Detection of JumonjiC Histone Demethylase and Dioxygenase Activity
JumonjiC domain-containing histone lysine demethylases (JMJCs) play a pivotal role in determining the epigenetic status of the genome by counteracting the activities of histone lysine methyltransferases. These enzymes act as erasers by catalyzing the removal of methyl marks from specific lysine sites in histones, leading to either transcriptional repression or activation of target genes.
The Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay rapidly detects succinate formation in JumonjiC histone demethylase and Fe(II)/α-ketoglutarate-dependent dioxygenase reactions. The assay uses the reaction product, succinate, to form ATP, which drives a bioluminescent reaction to produce a signal proportional to the original demethylase/hydroxylase activity.

Specifications

{"type": "table", "rows": [{"name": "Succinate, 10mM", "conditions": {"Part #": "V897A", "Size": "1 × 50μl"}}, {"name": "Succinate-Glo™ Buffer", "conditions": {"Part #": "V847A", "Size": "1 × 5ml"}}, {"name": "Succinate-Glo™ Solution", "conditions": {"Part #": "V894A", "Size": "1 × 50μl"}}, {"name": "ATP Detection Buffer", "conditions": {"Part #": "V895A", "Size": "1 × 10ml"}}, {"name": "Acetoacetyl-CoA, 100X", "conditions": {"Part #": "V896A", "Size": "1 × 100μl"}}, {"name": "ATP Detection Substrate", "conditions": {"Part #": "V898A", "Size": "1 × 1 vial"}}]}

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