Promega, V8345, IdeS Protease and IdeZ Protease

Cleavage Specificity of IdeS and IdeZ Proteases
IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading proteases that cleave with high specificity.
IdeS Protease is derived from Streptococcus pyogenes . It is an engineered, recombinant protease overexpressed in E. coli . IdeS Protease cleaves IgG at a single site below the hinge region, yielding F(ab') 2 and Fc fragments as shown here.
IdeZ Protease is derived from Streptococcus equi subspecies zooepidemicus . It is also an engineered, recombinant protease overexpressed in E. coli . Like IdeS, IdeZ Protease specifically cleaves IgG molecules below the hinge region, yielding F(ab') 2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.