Promega, V8500, MagneHis™ Protein Purification System

Name: MagneHis™ Protein Purification System
Brand: Promega
SKU: V8500

Magnetic Purification of Polyhistidine- or HQ-Tagged Proteins from, No centrifugation or vacuum required after cell lysis, Particles are compatible with a variety of common buffers, Binding capacity up to 1mg of polyhistidine-tagged protein per 1ml of particles, Configuration: 65 reactions

Catalog Number: V8500

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Category

Protein Purification Columns

Pack Size1 vial

TagsProtein Purification Protein Purification Kits

Description

Purify Polyhistidine (His-) or HQ-Tagged Proteins
MagneHis™ Protein Purification System provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP.
MagneHis™ Ni-Particles are compatible with a variety of common buffers. Binding capacity is approximately 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.
Note: This product requires the use of a magnetic stand.
For more information, see the Protocols & Applications Guide .
Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
High-throughput purification of His- or HQ-tagged proteins using automated platforms.
Purification of His-tagged proteins from insect or mammalian cell lysates.
Pull-down of His-tagged proteins for protein:protein interaction studies.
Purification of His-tagged methionyl tRNA synthetase.

Specifications

{"type": "table", "rows": [{"name": "MagneHis™ Binding/Wash Buffer", "conditions": {"Part #": "V851A", "Size": "1 × 50ml", "Available Separately": ""}}, {"name": "MagneHis™ Elution Buffer", "conditions": {"Part #": "V852A", "Size": "1 × 10ml", "Available Separately": ""}}, {"name": "MagneHis™ Ni-Particles", "conditions": {"Part #": "V854A", "Size": "1 × 2ml", "Available Separately": "View Product"}}, {"name": "FastBreak™ Cell Lysis Reagent, 10X", "conditions": {"Part #": "V857A", "Size": "1 × 15ml", "Available Separately": "View Product"}}, {"name": "DNase I (lyophilized)", "conditions": {"Part #": "Z358B", "Size": "1 × 1 vial", "Available Separately": "View Product"}}]}

Ordering

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