Promega, V8571, FastBreak™ Cell Lysis Reagent

Efficient Lysis of E. coli Cultures; No Centrifugation or Mechanical Disruption Needed
FastBreak™ Cell Lysis Reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ Protein Purification System. This product is suitable for both manual and automated protocols.
Eliminate centrifugation and mechanical disruption with this easy-to-use, add and incubate reagent, compatible with manual and robotic platforms.
Manual purification of a 6X His-tagged fusion protein using FastBreak™ Cell Lysis Reagent and the MagneHis™ Protein Purification System. Lane 1, bacterial cell lysate expressing 6X His-tagged firefly luciferase lysed by the addition of FastBreak™ Reagent; lane 2, postlysis cell supernatant demonstrating that the protein is solubilized; lane 3, flowthrough of cell lysate after the addition of MagneHis™ Ni-Particles; lane 4, eluted 6X His-tagged firefly luciferase; lane 5, Broad Range Protein Molecular Weight Markers (Cat.# V8491).