Promega, VA1092, GDP-Glo™ Glycosyltransferase Assay

Detects Low Nanomolar Amounts of GDP; Ideal for Low-Activity Glycosyltransferases
The sensitivity of the GDP-Glo™ Glycosyltransferase Assay means it can detect low GDP concentrations with high dynamic range. Therefore, whether assaying for a low-activity glycosyltransferase whose sugar transfer rate is low or using a low concentration of enzyme that produces a small amount of GDP, you can obtain a high signal-to-background ratio using the assay.
The data below show the linearity and sensitivity of the GDP-Glo™ Glycosyltransferase Assay. Here, a GDP standard curve was prepared over the indicated concentration range in 25µl of 1X glycosyltransferase reaction buffer in a solid white 96-well plate. GDP-Glo™ Glycosyltransferase Assay was performed using 25µl of GDP Detection Reagent at room temperature. Luminescence was recorded using a GloMax® luminometer. Values represent the mean of four replicates. The table shows luminescence measured 1 hour after adding the GDP Detection Reagent, and signal-to-background ratio calculated for each concentration of the standard curve.