Description
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The FIP1L1-PDGFRα Kinase Enzyme System contains:
FIP1L1-PDGFRα Kinase, 10μg [Human, recombinant fusion; FIP1L1 (1–265)-PDGFRα (579–end)]. MW: ~130kDa
Poly (Glu 4 , Tyr 1 ) Peptide Substrate (1mg/ml)
5X Reaction Buffer, 2.5 MnCl 2 0.1M DTT
Recombinant human FIP1L1-PDGFRα, the fusion protein [FIP1L1 (1–265)-PDGFRα (579–end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FIP1L1 or factor interacting with PAPOLA and CPSF1 is a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRα fusion gene encodes a constitutively activated tyrosine kinase that transforms hematopoietic cells and is inhibited by imatinib in which fusion of the PDGFRα and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome.
The FIP1L1-PDGFRα Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
