Description
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The EIF2AK4 Kinase Enzyme System contains:
EIF2AK4 Kinase, 10μg (Human, recombinant; amino acids 192–1024). MW: ~132kDa
RS Repeat Peptide Substrate (GRSRSRSRSRSRSRSR) (1mg/ml)
5X Reaction Buffer, 0.1M DTT
Recombinant human EIF2AK4 (amino acids 192–1024) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EIF2AK4 or eukaryotic translation initiation factor 2 alpha kinase 4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. The EIF2AK4 eIF2-alpha kinase regulates fatty-acid homeostasis in the liver during deprivation of an essential amino acid.
The EIF2AK4 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
