Description
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The DGKZ Kinase Enzyme System contains:
DGKZ Kinase, 10μg (Human, recombinant, full-length); MW: ~132kDa
Dilauroyl-sn-glycerol Substrate, 10mM
5X Reaction Buffer; DTT, 0.1M; Lipid Dilution Buffer II
Recombinant full-length human DGKZ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Diacylglycerol kinase zeta (DAG kinase zeta) is a member of class IV diacylglycerol kinase family, which contains two zinc fingers, an ATP binding site, and four ankyrin repeats with the MARCKS phosphorylation site, and exhibits the preference for 1,2-diacylglycerols but not for long chain diacylglycerols. DGK zeta regulates Ras activation via terminating the diacylglycerol signaling induced RasGRP.
The DGKZ Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
