Promega, W1001, ECL Western Blotting Substrate

Name: ECL Western Blotting Substrate
Brand: Promega
SKU: W1001

Chemiluminescent Substrate to Detect Horseradish Peroxidase (HRP) Conjugates on Immunoblots, Detect picogram levels of protein with minimal background, Non-radioactive, No optimization required; easily switch from other entry-level ECL substrates, Configuration: 250ml

Catalog Number: W1001

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Category

ECL Substrates

TagsProtein Detection Protein Labeling

Description

Highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.
No optimization required; easy to switch from other entry-level ECL substrates.
Western blotting
Dot blotting
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells. The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng) as quantitated via ELISA. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate, secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and with 1-minute exposure.

Specifications

{"type": "table", "rows": [{"name": "Peroxide Solution", "conditions": {"Part #": "W100A", "Size": "1 × 125ml"}}, {"name": "Luminol Enhancer Solution", "conditions": {"Part #": "W101A", "Size": "1 × 125ml"}}]}

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