Promega, W1620, Lumit® Anti-Tag Protein Interaction Reagents

Name: Lumit® Anti-Tag Protein Interaction Reagents
Brand: Promega
SKU: W1620

Solution-Based Approach to Detect Protein Interactions of Tagged Proteins, Fast and sensitive detection of protein interactions in 30–120 minutes, No wash, transfer or immobilization steps, Reagents are anti-tag antibodies (and streptavidin) that are conjugated with Lumit® LgBiT and SmBiT, Tags include 6His, GST, DYKDDDDK (recognizes FLAG®), human IgG and biotin, Application Notes available below, These reagents are used with, Lumit® Detection Reagent A, Configuration: Lumit® Anti-GST/LgBiT and

Catalog Number: W1620

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Category

Biotin-Conjugated Secondary Antibodies

TagsImmunoassays Lumit® Immunoassays for Research

Description

Scalable Assay Reagents to Detect Protein Interactions of Tagged Proteins
Lumit® Anti-Tag Protein Interaction Reagents allow researchers to measure protein-protein or protein-small molecule interactions involving tagged proteins in a homogeneous (no-wash) assay format.
The assay combines immunodetection and NanoLuc® Binary Technology (NanoBiT). NanoBiT is a structural complementation reporter ideal for interaction studies. The NanoBiT® System is composed of two subunits: Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide). These subunits can be expressed as recombinant fusions or chemically conjugated to antibodies or other target proteins of interest. The LgBiT and SmBiT subunits have been optimized for stability and minimal self-association due to their weak affinity (K d = 190µM). When LgBiT and SmBiT are brought into proximity, they form a functional enzyme that generates luminescence in the presence of its substrate.

Specifications

{"type": "table", "rows": [{"name": "Lumit® Anti-GST-LgBiT", "conditions": {"Part #": "W162A", "Size": "1 × 20μl"}}, {"name": "Lumit® Anti-GST-SmBiT", "conditions": {"Part #": "W163A", "Size": "1 × 20μl"}}]}

Ordering

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