Promega, W1621, Lumit® Anti-Tag Protein Interaction Reagents

Scalable Assay Reagents to Detect Protein Interactions of Tagged Proteins
Lumit® Anti-Tag Protein Interaction Reagents allow researchers to measure protein-protein or protein-small molecule interactions involving tagged proteins in a homogeneous (no-wash) assay format.
The assay combines immunodetection and NanoLuc® Binary Technology (NanoBiT). NanoBiT is a structural complementation reporter ideal for interaction studies. The NanoBiT® System is composed of two subunits: Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide). These subunits can be expressed as recombinant fusions or chemically conjugated to antibodies or other target proteins of interest. The LgBiT and SmBiT subunits have been optimized for stability and minimal self-association due to their weak affinity (K d = 190µM). When LgBiT and SmBiT are brought into proximity, they form a functional enzyme that generates luminescence in the presence of its substrate.