Description
Polyclonal secondary antibody raised in goat, immunoaffinity-purified using immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme.
The Anti-Mouse IgG (H+L), HRP Conjugate , antibody binds to both heavy and light chains for all mouse IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution. A starting working dilution of 1:2,500 is suggested for most Western blot, dot blot and ELISA applications. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.
We can readily accommodate large-scale custom orders. Please inquire at: www.promega.com/custom/
Applications
Western blotting.
Enzyme-linked immunosorbent assay (ELISA).
Dot blotting.
References
Hu, D. et al. (2012) Novel insight into KLF4 proteolytic regulation in estrogen receptor signaling and breast carcinogenesis. J. Biol. Chem. 287 , 13584–97.
Hesse, E. et al. (2010) Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J. Cell Biol. 191 , 1271–83.
Massaad, C.A., Washington, T.M., Pautler, R.G. and Klann, E. (2009) Overexpression of SOD-2 reduces hippocampal superoxide and prevents memory deficits in a mouse model of Alzheimer's disease. Proc. Natl. Acad. Sci. USA 106 , 13576–81.
Berkowitz, O., Jost, R., Pollmann, S. and Masle, J. (2008) Characterization of TCTP, the translationally controlled tumor protein, from Arabidopsis thaliana . Plant Cell 20 , 3430–47.
Robertson, J.B., Stowers, C.C., Boczko, E. and Johnson, C.H. (2008) Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast. Proc. Natl. Acad. Sci. USA 105 , 17988–93.
Enzyme-linked immunosorbent assay (ELISA) for β-actin. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; ELISA developed with TMB One Solution (Cat.# G7431).
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells. The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of total protein (µg). Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and 1-minute exposure.
