Description
BioSprint DNA Blood Kits provide all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots and buccal swabs using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.
DNA can be successfully purified from a variety of sample types (see table “Typical yields from a range of sample types”). Typical yields from a range of sample types Sample type Amount of sample DNA (µg) BioSprint 15 BioSprint 96 Muscle, bovine 25 mg 16.2 ± 2.5 13.5 ± 1.5 Heart, bovine 25 mg 5.9 ± 2.6 3.4 ± 0.6 Spleen, bovine 25 mg 69.1 ± 23.6 59.1 ± 4.8 Lung, bovine 25 mg 13.8 ± 7.2 14.7 ± 5.5 Liver, bovine 25 mg 77.8 ± 29.4 74.0 ± 22.3 Kidney, bovine 25 mg 26.2 ± 18.8 33.5 ± 5.4 Ear, sheep 30 mg 20.31 ± 1.8 – Tail, mouse 1.2 cm (~25 mg) 32.7 ± 4.6 30.9 ± 4.5 HL-60 cultured cells 2 x 10 6 cells 10.1 ± 4.7 9.6 ± 5.6 Blood, human (5–7 x 10 6 cells per ml) 200 µl 4.5–9.0 4.5–9.0 Blood, bovine 200 µl 6.2–8.0 4.4–7.0 Blood, sheep 200 µl 5.6–11.2 1.2–8.0 Blood, pig 200 µl 4.5–9.0 4.5–9.0 Blood, dog 200 µl 6.7–12.3 6.9–16.1 Blood, cat 100 µl 4.4–8.3 2.9–6.3 Blood, mouse 100 µl 2.0–8.0 1.5–3.0 Blood, rat 100 µl 1.0–4.0 1.0–3.0 Blood, bird 10 µl* 15.0 ±6.3 – Blood, fish 5 µl* 7.1–10.0 – Dried blood spots, 903 Specimen Collection Paper 1 punch (6 mm [1/4 inch] diameter) 0.30 0.10 Dried blood spots, FTA Card 1 punch (6 mm [1/4 inch] diameter) 0.12 0.05 Buccal swabs 1 swab 0.8–2.0 0.8–2.0 Genomic DNA was purified from the indicated samples. DNA was eluted in 200 µl Buffer AE. * Sample diluted to 200 µl. Purified DNA is up to 50 kb in size (see figures "Purification of high-quality genomic DNA from mouse tail "and "Reproducible purification of DNA from sheep ear tissue"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure "High PCR performance of purified DNA").
BioSprint DNA Blood Kits are used with the BioSprint 15 or BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from cells, tissue and blood or blood-related products. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.
Genomic DNA can be purified from cells, tissue and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables. For purification of genomic DNA from tissue, dried blood spots or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with BioSprint DNA Blood Kits).
