| Application Area | Cell dissociation |
|---|
| Origin | Animal-Component Free |
|---|
| Serum Free | Yes |
|---|
| Adherence/Suspension | Adherent |
|---|---|
| Formulation | With EDTA |
| Delivery Condition | Sterile |
|---|
| Volume | 500 mL |
|---|
Answer:No. The reagent uses recombinant trypsin and is specified as animal component-free.
Answer:EDTA helps accelerate detachment by reducing calcium- and magnesium-dependent cell adhesion.
Answer:The specified bottle contains 500 mL of sterile, ready-to-use solution.
Sartorius Recombinant Trypsin-EDTA Solution is a sterile, ready-to-use, animal component-free reagent for detaching adherent cells from culture vessels and substrates. Recombinant trypsin cleaves proteins involved in cell attachment, while EDTA supports efficient dissociation by chelating divalent ions. The defined preparation provides an alternative to animal-derived trypsin solutions and is designed for sensitive cell types including human pluripotent stem cells and mesenchymal stem cells. The 500 mL format supports repeated cell-culture handling and passaging. Exposure time and subsequent enzyme neutralization should be optimized for the cell type and culture conditions.
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