Description
Incucyte®Nuclight Lentivirus 2.0 offers a sophisticated, easy-to-use method for nuclear labeling and real-time quantitation of cell proliferation and viability.
Incucyte®Nuclight Lentivirus Reagents enable efficient, non-perturbing, nuclear labeling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times.
Incucyte®Nuclight Lentivirus Reagents provide homogenous expression of a nuclear restricted mEGFP (green fluorescent protein), mCherry2 (red fluorescent protein) or iRFP713 (NIR fluorescent protein) driven by EF1α promoter in your choice of primary, immortalized, dividing or non-dividing cells. These reagents are ideal for generating stable cell populations or clones using puromycin or bleomycin selection. Furthermore, the Incucyte®Nuclight Lentivirus Reagents can be combined with the Incucyte®Confluence Metric, Incucyte®Annexin V Dyes, Incucyte®Caspase 3/7 Dyes or Incucyte®Cytotox Dyes for multiplexed measurements of apoptosis, cell proliferation (label-free or fluorescently labeled) or cytotoxicity in every assay well.
Presentation, Storage and Stability
Incucyte®Nuclight Lentivirus Reagents are supplied as 0.6 mL vials of 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles suspended in DMEM. Lentiviruses should be handled with utmost care and used only by experienced individuals or under direct supervision. Incucyte®Nuclight Lentivirus Reagents are stable for 6 months from date of receipt when stored at -80°.
Recommended Use
Incucyte®Nuclight Lentivirus can be prepared in full media and added directly to plated cells. We recommend an MOI of 3 to 6, depending on the infected cell type. Optional materials such as the cationic polymer Polybrene®can be added to enhance transduction efficiency.
Post-infection, stable cells may be generated using appropriate antibiotic selection. Please refer to our Product Guide for more information regarding recommended use, protocols, guides and safety information.
