Description
CIMmultus®Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom-designed housing is a single-piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
The PrimaS® HR line is a new generation of pre-packed chromatographic monolithic products developed to meet the most demanding separation and purification needs. HR stands for High Reproducibility between batches and different scales.
Features
Multimodal chromatography ligand that combines elements of hydrogen bonding with weak anion exchange chromatography.
Selected to meet the need for highly reproducible purification processes (e.g., separation of full AAV capsids from empty AAV capsids)
Column release criteria based on the AAV8 elution profile.
AAV Binding at neutral or closer to neutral pH compared to QA-based columns: Multimodal properties of PrimaS® HR allow binding of AAV in neutral or closer to neutral pH values compared to QA-based columns.
Reusability across multiple cycles reduces cost and supports high-throughput needs.
Endotoxin Removal: Provides superior reduction of endotoxin compared to strong anion-exchangers (e.g. QA).
cGMP-compliant: suitable for clinical and commercial production.
Benefits
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields
