Thermo Fisher, 0010057DG, Anti-Clumping Agent, 100 mL

Name: Anti-Clumping Agent, 100 mL
Brand: Thermo Fisher
SKU: 0010057DG

Quantity: 100 mL, Unit Size: Each

Catalog Number: 0010057DG

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Category

Non-Animal Origin Dissociation Reagents

Pack Size100 mL

TagsCell Culture Media Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements Additional Cell Culture Reagents and Supplements

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mL
Unit Size: Each
GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.
• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin free (AOF) and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and deliverySimple and Gentle Cell DissociationCell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping” state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.Reduced Clumping, Increased Viability, Improved Protein ExpressionReduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.For actual cell dissociation, we recommend our animal origin-freeTrypLE™ products.Use Anti-Clumping Agent after TransfectionAnti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.Animal Origin Free and Chemically Defined ComponentsBecause Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.Convenient and Customizable PackagingIn addition to this 20 ml size, Anti-Clumping Agent is also available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking the 'Customize this Product' link under the 'Add to Cart' button above or by contacting us [email protected] Information and SupportPlease review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to ourTechnical Servicesgroup.For Research Use Only. Not for use in diagnostics procedures.

Specifications

General

Cell Line	CHO, Insect Cells, 293 (HEK)
Cell Type	Mammalian Cells
Culture Type	Suspension Cell Culture
Format	Bottle(s)
Product Line	Gibco
Quantity	100 mL
Shipping Condition	Room Temperature
Source	Animal origin-free
Classification	Chemically-defined
Form	Liquid
Product Type	Anti-clumping Reagent
pH	6 to 8
Unit Size	Each

FAQs

Question: What is Anti-Clumping Agent used for?

Answer:It is used to reduce cell aggregation and help maintain more uniform suspension cultures.

Question: Which cell types can be used with the reagent?

Answer:The reagent can be used with suspension systems including CHO, HEK293 and insect-cell cultures.

Question: Is the formulation animal-origin-free?

Answer:Yes. The formulation is chemically defined and animal-origin-free, without proteins, enzymes, hydrolysates or undefined components.

Question: What volume is supplied?

Answer:This configuration contains 100 mL.

Question: How should the reagent be stored?

Answer:Store at 2–8°C.

Application Note

Anti-Clumping Agent is a concentrated liquid cell-culture reagent used to reduce aggregation in suspension cultures and help maintain a more uniform single-cell suspension. The formulation is chemically defined and animal-origin-free, with no proteins, enzymes, hydrolysates or undefined components. It can be used with suspension-adapted mammalian and insect cell systems, including CHO, HEK293 and insect-cell cultures, when clumping limits cell handling or culture performance. The reagent is particularly useful during expansion, passaging and other workflows where aggregated cells can interfere with cell counting, sampling or consistent culture operation. This configuration contains 100 mL, has a pH range of about 6–8 and should be stored at 2–8°C. It is intended for research use only.

Ordering

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