Thermo Fisher, 10622D, Exosome-Human CD81 Flow Detection Reagent (from cell culture)

Name: Exosome-Human CD81 Flow Detection Reagent (from cell culture)
Brand: Thermo Fisher
SKU: 10622D

Quantity: 2 mL, Unit Size: Each

Catalog Number: 10622D

Pricing

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Category

Cell Sorters

Pack Size2 mL

TagsCell Analysis Products Microspheres

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 2 mL
Unit Size: Each
Cell culture media must first be enriched for exosomes using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Exosome-Human CD81 Flow Detection Reagent (from cell culture) allows easy visualization of exosomes by flow cytometry using this magnetic separation technology. Free exosomes alone are too small to be detected by most flow cytometry instruments. The 2.7 μm Dynabeads magnetic beads allow for a clear and defined flow analysis.
• Minimal hands-on time for specific exosome capture and analysis
• Clear and defined flow analysis in less than 1 hour hands-on time
• Study expression of specific exosome subset markers
• Protocol is easily scalable
• Recent studies suggest that exosome/ECV population consists of several subsets, possibly with different functions
• Using immuno-affinity based Dynabeads magnetic separation technology, these subsets can be selectively captured and studied using flow cytometry and/or electron microscopy
• Dynabeads technology avoids loss of material due to handling and centrifugation typically experienced with other isolation and analysis methodsMix thoroughly to ensure that magnetic beads do not settle in the sample tube. A mixer which provides both tilting and rotation for optimal mixing is recommended.

Specifications

General

Product Line	Dynabeads
Quantity	2 mL
Sample Type	Cell Cultures
Diameter (Metric)	2.7 μm
Product Type	Flow Detection Reagent
Unit Size	Each

FAQs

Question: Which extracellular vesicle marker is targeted?

Answer:The bead-based detection reagent captures human CD81-positive extracellular vesicles.

Question: Why are Dynabeads used for flow cytometry of exosomes?

Answer:Captured vesicles bind to approximately 2.7 µm magnetic beads, which can be analyzed by conventional flow cytometry.

Question: What is the pack volume and recommended storage range?

Answer:The reagent is supplied in 2 mL and stored at 2–8°C.

Application Note

Invitrogen Exosome-Human CD81 Flow Detection Reagent provides a magnetic-bead-based approach to detecting CD81-positive extracellular vesicles from enriched cell culture medium. The reagent uses Dynabeads technology to capture vesicles bearing the CD81 tetraspanin marker. Bound vesicles are presented on approximately 2.7 µm magnetic particles, making bead-associated material accessible to conventional flow cytometry analysis even though free exosomes fall below the detection range of many cytometers. The workflow begins with enrichment of extracellular vesicles from culture supernatant, followed by immunoaffinity capture and staining or detection. The reagent is supplied in a 2 mL format and is stored at 2–8°C. CD81 positivity defines the selected vesicle population and does not, by itself, demonstrate the presence or purity of every possible exosome subtype.

Ordering

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