Thermo Fisher, 11965092, DMEM, high glucose
Quantity: 500 mL, Unit Size: Each
Catalog Number: 11965092
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Category
Pack Size500 mL
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| Cell Line | HeLa, 293, Cos-7, and PC-12 |
|---|---|
| Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Glucose Concentration | 4500 mg/L |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
| Quantity | 500 mL |
| Shelf Life | 12 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin-free |
| Form | Liquid |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine |
| Without Additives | No HEPES |
| Unit Size | Each |
Answer:A 500 mL bottle of ready-to-use 1× DMEM high-glucose liquid medium.
Answer:No. This formulation contains high glucose and L-glutamine but is supplied without sodium pyruvate.
Answer:4.5 g/L D-glucose; the formulation also contains 4 mM L-glutamine and phenol red.
Gibco Dulbecco’s Modified Eagle Medium (DMEM), high glucose, is a ready-to-use 1× liquid basal medium for culturing suitable mammalian cell lines under validated conditions. This 500 mL formulation contains 4.5 g/L D-glucose, L-glutamine and phenol red, and it does not contain sodium pyruvate. The included amino acids, inorganic salts and vitamins support cell culture when combined with the supplements, buffering system and incubation conditions required by the particular cell model. The L-glutamine concentration in the formulation is 4 mM, while sodium bicarbonate provides part of the buffering system. High-glucose DMEM is not interchangeable with low-glucose DMEM, sodium-pyruvate-containing variants or GlutaMAX formulations without considering the cell line and experiment. Maintain aseptic technique and follow the product storage instructions and cell-line-specific serum or serum-free supplementation requirements.
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