Thermo Fisher, 12491015, Advanced DMEM

Quantity: 500 mL
Unit Size: Each
This Advanced DMEM is manufactured as follows:With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol RedWithout: L-glutamineThe complete formulation is available.Using Advanced DMEMAdvanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO_2 environment to maintain physiological pH.