Thermo Fisher, 15224025, T4 DNA Ligase (1 U/μL), 500 U

Quantity: 500 U
Unit Size: Each
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.
• Optimizes ligation in 5 minutes
• Source: Purified fromE. colilambda lysogen NM989
• Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
• Unit definition: One unit catalyzes exchange of 1nmol^32P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
• Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl_2, 10mM DTT, 66μM ATP, 3.3μM^32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°CChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning