Thermo Fisher, 17105041, Dispase II, powder

Dispase II powder is used for gentle cell and tissue dissociation, isolation of primary cells and routine passaging where preservation of cell-membrane integrity is important. Dispase II is a neutral amino-endopeptidase that preferentially hydrolyzes peptide bonds adjacent to nonpolar amino-acid residues, sequences that occur frequently in collagen. The relatively mild proteolytic action supports separation of tissues and cell aggregates at physiological temperature and pH. This configuration contains 5 g of lyophilized, non-sterile powder produced from Bacillus polymyxa, with activity specified above 0.5 units/mg. The reagent can also help reduce clumping in suspension cultures. The dry product is stored at 2–8°C protected from light, while reconstituted solutions can be frozen for longer-term storage when prepared for laboratory use.