Thermo Fisher, 18011015, Bacterial Alkaline Phosphatase

Quantity: 2500 U
Unit Size: Each
BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extraction
• Source: Purified fromE. coliC90
• Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determined
• Unit Definition: One unit hydrolyzes 1nmol of ATP in 30 min. at 37°C
• Unit Reaction Conditions: 10mM Tris-HCl (pH 8.0), 1.5mM [γ-^32P]ATP, and enzyme in 50μL for 30 min. at 37°CDephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning