Description
Quantity: 25 reactions kit
Unit Size: Each
Features of GST Tag Protein Interaction Pull-Down Kit:
• GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins from many different sources
• Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resinApplications:
• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information fromin vitrotranscription/translation lysatesYou provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.Related ProductsPierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)