Thermo Fisher, 25530049, Proteinase K Solution (20 mg/mL), RNA grade

Quantity: 5 mL
Unit Size: Each
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C
• Protein Filter: PApplicationsRemoval of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.Performance and quality testingEndodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.Unit definitionOne mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.