Thermo Fisher, 26178, Pierce™ Rapid Antibody Isotyping Kit - Mouse

Quantity: 10 Tests
Unit Size: Each
The kits are composed of small cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kits determine mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM.The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.Highlights:
• Long shelf life– stable for more than 1 year at room temperature
• Single-step– add diluted antibody sample to the loading-well of cassette
• Fast– within 5 minutes a band appears that indicates the antibody isotype
• Sensitive– detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL
• Specific– assay does not crossreact with fetal bovine serum (FBS)
• Reliable– results are similar to the standard ELISA-based isotyping assays Includes:Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent