Thermo Fisher, 28103-104030, Hypersil™ BDS C18 Columns

Hypersil™ BDS C18 Column, 100 x 4.0 mm, 3 µm is used for robust reversed-phase HPLC separations in routine analytical and QC/QA workflows. It contains an endcapped, base-deactivated C18 stationary phase on fully porous silica and is configured as a 4.0 mm inner-diameter × 100 mm analytical column with 3 µm particles. The Hypersil BDS surface treatment reduces silanol interactions and supports improved peak symmetry for basic compounds while maintaining suitability for acidic and neutral analytes. The phase has a 130 Å pore size and 11% carbon load, with an operating pH range of 2–9, a maximum temperature of 60 °C, and a pressure rating of 5800 psi (400 bar). This combination supports routine assays that need a conventional USP L1 C18 phase with base-deactivated silica and consistent retention characteristics. The specific 100 mm × 4.0 mm format and 3 µm packing allow direct selection for methods that define column geometry and particle size as part of the chromatographic conditions.