Description
Quantity: 50 mL
Unit Size: Each
Features of CaptureSelect CD81 Magnetic Agarose Beads include:
• One-step human CD81-expressing exosome purification with high purity from crude cell clarified supernatant, TFF-concentrated cell lysates, and blood plasma
• Compatible with manual and automated workflows, e.g., KingFisher instruments
• Short operation time
• No sample dilution required during isolation; repetitive sample loading feasible
• Relatively mild elution at pH 9, resulting in efficient release of exosomes from the magnetic beads.
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (V_HH) created by a proprietary technology. The V_HH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in yeast Saccharomyces cerevisiae by a production process free of any animal components (animal origin-free).Main characteristicsMatrix: 6% cross-linked magnetized agaroseAverage particle size: 25-30 μmSuspension: 25% slurryLigand: CaptureSelect CD81 affinity ligandBinding capacity: estimated at ≥ 1 x 10^10 EVs /mL of magnetic bead slurryElution conditions: 50 mM Tris, 1 M arginine, pH 9Formulation buffer: Tris-buffered saline, 0.05% Tween 20, 0.05 % sodium azide