| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
|---|---|
| Kit Type | 5 Pack |
| Passive Reference Dye | ROX (Pre-mixed) |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Product Line | TaqMan |
| Product Type | Universal PCR Master Mix |
| Quantity | 5 x 5 mL |
| Sample Type | DNA (Genomic), cDNA |
| Volume | 55 mL |
| Detection Method | Primer-probe |
| For Use With (Application) | Gene Expression |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Reaction Speed | Standard |
| Unit Size | Each |
Answer:It is supplied as a 2X master mix.
Answer:The package contains five 5 mL tubes.
Answer:The configuration provides enough master mix for approximately 1000 reactions.
TaqMan™ Universal PCR Master Mix is a 2X reagent mixture for probe-based real-time PCR using TaqMan 5′-nuclease chemistry. This configuration contains five 5 mL tubes and provides sufficient reagent for approximately 1000 reactions at a 50 µL final reaction volume. The mix contains AmpliTaq Gold™ DNA Polymerase, uracil-DNA glycosylase, dNTPs with dUTP, premixed ROX passive reference dye, and optimized buffer components. It is intended for DNA and cDNA targets in applications such as gene-expression analysis, genotyping, and targeted quantitative PCR. The integrated UNG system supports carryover-contamination control when dUTP-containing amplicons are present. The product uses standard cycling conditions and should be stored at 2–8 °C protected from light.
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