Description
Quantity: 5 L
Unit Size: Each
The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids. Highlights:
• Cost-effective– purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY
• High purity– method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)
• Easy to use– simple precipitation method purifies chicken IgY without affinity columns
• Flexible protocol– eggs can be harvested and quickly stored in buffer for purification at a later date
• Convenient– use eggs directly out of refrigerator; no need to equilibrate them to room temperature Includes:Egg separator, delipidation reagent and IgY precipitation reagent
