| Column Format | Immobilized enzyme |
|---|---|
| Column Type | Immobilized enzyme in PEEK cartridge |
| Description | SMART Digest Pepsin/Protease XIII Dual Enzyme Column |
| Diameter | 2.1 mm |
| Flow Rate | 10 to 500 μL/min |
| For Use With (Application) | HDX-MS analysis |
| Length | 30 mm |
| Max. Pressure | 2500 psi (170 bar) |
| Mobile Phase Compatibility | Phosphate buffer pH 4 |
| Operating Temperature Range | 4 °C to 50 °C |
| Packing Material | Hydrophilic/coated DVB |
| Particle Size | ˜20 μm |
| Pore Size | ° 1000 angstroms |
| Stability | 1 year |
| Stationary Phase | Immobilized enzyme |
| Storage Temperature | RT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6) |
| Technique | BioLC |
| Weight | 8 g |
| Cleavage | Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0. <br /><br /> Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH. |
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| Enzyme | Pepsin & Protease XIII |
|---|
| Type | SMART Digest Pepsin/Protease XIII Dual Enzyme column |
|---|
| pH | 2 to 6 |
|---|
| Unit Size | Each |
|---|
