Description
Quantity: 1 mL
Unit Size: Each
Features of this affinity matrix include:
• Single-step purification of C-terminal tagged proteins directly from complex source materials
• Mild elution, making it suitable for pH sensitive proteins
• Elution possible at pH 7.0
• Works in the presence of serum (bovine/horse)
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristicsMatrix: agarose-based, epoxide-activatedAverage particle size: 65 ± 10 μmLigand: CaptureSelect C-tagXL affinity ligandBinding capacity: 400 nmol/mL resin, depending on flow rate, column height, and contact timeElution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine, pH 3.0Neutral: 20 mM Tris, 2.0 M MgCl_2, pH 7 or 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol or 20 mM Tris, 2 mM S-E-P-E-AColumn dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)Max. operation pressure: 2 barFormulation buffer: 20% (v/v) ethanol
