Thermo Fisher, 71194400UG, HotStart-IT Binding Protein

Quantity: 400 μg (200 x 25 μL rxns)
Unit Size: Each
Features
• Room temperature reaction setup
• Minimizes amplification of non-specific products and primer-dimers (see figure)
• Ideal for complex templates and multiplex reactions
• No extensive heating step required
• Free from detectable non-specific nucleases
• Technology is portable to a polymerase of choiceThis primer-sequestration technique effectively blocks DNA synthesis from mis-priming events at lower temperatures. Following the initial denaturation step during PCR, the protein is inactivated, and the primers are free to participate in the amplification reaction.HotStart-IT Binding Protein performs well in many standard PCR reaction buffers and has been designed for PCR applications that demand high specificity and sensitivity. PCR with Taq DNA Polymerase and HotStart-IT Binding Protein shifts production of primer-dimers to a specific target of 306 bp from 1 ng of human genomic DNA relative to Taq DNA Polymerase alone.Application
• Hot-Start PCR amplificationNotes
• One microgram of HotStart-IT Binding Protein sequesters about 5 pmol of primers.
• HotStart-IT Binding Protein is sourced from recombinant protein expressed inE. coli.