Description
Quantity: 2 x 100 μL
Type: 1-step Human High-yield Mini IVT Kit
Unit Size: Each
The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector. Highlights:
• High expression– up to 750μg/mL of expressed protein
• Reproducibility– low variability between experiments
• Fast– express high levels of protein with an overnight incubation
• Functional– obtain more active protein than with bacterial protein expression systems 1-Step Protein Expression:
• Streamlined reaction– requires only five components
• Easy-to-use– transcription and translation is performed in one reaction step
• Adapt for labeling– amenable to incorporation of heavy or unnatural amino acids Includes:Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes Requires:30°C incubator or water bath Recommended for:Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production
