Thermo Fisher, A13868.14, 3,3',5,5'-Tetramethylbenzidine, 98%

Quantity: 25 g
Unit Size: Each
General Description
• 3,3',5,5'-Tetramethylbenzidine, also known as 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl or TMB, is a colorimetric substrate for detection of horseradish peroxidase-labeled probes
• Oxidation of 3,3',5,5'-Tetramethylbenzidine results in a pale blue end product that can be detected at 370 or 655 nm. Sulfuric acid (H2SO4) is used to stop the oxidation reaction and stabilize the reading, resulting in a yellow color that can be read at 450 nmApplication
• 3,3',5,5'-Tetramethylbenzidine is used as a chromogenic visualization agent in enzyme-linked immunosorbent assays (ELISA) or immunohistochemistry (IHC) techniques
• 3,3',5,5'-Tetramethylbenzidine has been used to detect molecules in aqueous samples such as copper ion and hypochlorite