Thermo Fisher, A15005, pJTI™ R4 EXP CMV-TO EmGFP pA Vector

Name: pJTI™ R4 EXP CMV-TO EmGFP pA Vector
Brand: Thermo Fisher
SKU: A15005

Quantity: 100 μg, Unit Size: Each

Catalog Number: A15005

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Category

Molecular Identification Kits

Pack Size100 µg

TagsMolecular Biology Reagents and Kits DNA Vectors

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 μg
Unit Size: Each
The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green. Ensure the Success of Your Jump-In™ T-REx™ Retargeting ReactionsSuccessful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:
• Transfection efficiency
• Cell confluency
• Antibiotic selection conditions
• Quality and concentration of DNA
• Retargeting vector to integrase vector ratioWe strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.

Specifications

General

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Constitutive or Inducible System	Inducible
Delivery Type	Transfection
For Use With (Application)	Stable Cell Line Development, Targeted Integration, Regulated Expression
Inducing Agent	Tetracycline
Product Type	Mammalian Expression Vector
Quantity	100 μg
Reporter Gene	GFP (EmGFP)
Selection Agent (Eukaryotic)	Geneticin™ (G-418)
Vector	Jump-In Vectors
Product Line	Jump-In
Promoter	CMV/TO
Protein Tag	GFP (EmGFP)
Unit Size	Each

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