Description
Cell Line: CHO-S, SFM Adapted
Unit Size: Each
The ExpiCHO Expression System enables:
• Milligram to multigram yieldsof recombinant protein per liter of culture in 5–14 days post-transfection
• Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
• Transfection efficiency of > 90%for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Use of one-third to one-half less plasmid DNAthan other transfection reagents
• Flexible, reliable cultureand transfection protocols with multiple options that readily fit into current transient expression workflows
• Scalable expressionfor culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasksThe components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.All components of the system are animal origin–free.
