Thermo Fisher, A55242, ExoSAP-IT™ PCR Product Cleanup Reagent

Quantity: 200 μL, 100 Preps
Unit Size: Each
• ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product by hydrolyzing excess primers and nucleotides in a single step
• ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis
• The reaction setup is complete with one pipetting step, which is followed by two incubations
• The first incubation digests excess primer and dephosphorylates nucleotides
• The second, high-temperature incubation inactivates the enzymes
• Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination
• After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.