Thermo Fisher, A72127, Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Name: Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Brand: Thermo Fisher
SKU: A72127

Quantity: 100 Reactions, Product Type: Lyo-ready RPA Kit, Unit Size: Each, Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Catalog Number: A72127

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Category

One-Step RT-PCR Kits

Pack Size100 reactions

TagsMolecular Biology Reagents and Kits LAMP and Other Isothermal Amplification Technologies Isothermal Amplification Reagents

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 Reactions
Product Type: Lyo-ready RPA Kit
Unit Size: Each
Features of Lyo-ready RPA products
• Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
• Robust and specific RNA/DNA amplification
• Amplification from inhibitor-containing samples
• Fast target amplification in as little as 20 minutes
• Ability to easily optimize RPA or RT-RPA reactionsKit componentsThe Lyo-ready RPA Kit includes all required components for an RPA reaction:
• Lyo-ready T4 UvsX Protein—produced fromE. colicells bearing cloned uvsX gene derived from bacteriophage T4
• Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
• Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
• Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced fromE. colicells carrying cloned bacteriophage T4 gene 32
• Optimized reaction buffer, magnesium chloride, and dNTPs

Specifications

General

Detection Method	End point, real-time, lateral flow
Optimal Reaction Temperature	42°C
Reaction Time	Up to 20 min.
Reverse Transcriptase	SuperScript IV Reverse Transcriptase
Shipping Condition	Dry Ice
For Use With (Application)	RT-RPA
Form	Liquid
Polymerase	Lyo-ready Bst DNA Polymerase
Product Type	Lyo-ready RPA Kit
Quantity	100 Reactions
Research Category	Nucleic Acid Amplification
Unit Size	Each

Ordering

Notice

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