Thermo Fisher, AM1556, <i>mir</i>Vana™ PARIS™ RNA and Native Protein Purification Kit

Name: mir Vana™ PARIS™ RNA and Native Protein Purification Kit
Brand: Thermo Fisher
SKU: AM1556

Quantity: 40 preps, Unit Size: Each

Catalog Number: AM1556

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Category

Total RNA Isolation Kits

Pack Size40 preps

TagsMolecular Biology Reagents and Kits RNA Isolation and Purification Products

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 40 preps
Unit Size: Each
The mirVana™ PARIS™ Kit is for the purification of both native protein and RNA (including small RNA) from the same sample. Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolations of protein and separate large and small RNA fractions.How the kit works The mirVana PARIS Kit procedure begins with homogenization of samples with a special cell disruption buffer that includes nonionic detergent. Protein remains intact, so a portion of the lysate can be used directly for common protein analysis applications. The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both.
• Isolate small RNA-containing total RNA and native protein from the same sample
• Start with 100–10^7cultured cells or up to 100 mg of many mammalian tissues
• Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses
• Follow a simple, 30-minute procedure
• Use to correlate mRNA, miRNA or siRNA, and protein levelsNot high-throughput compatible (Manual)

Specifications

General

Final Product Type	Total RNA, Transcriptome RNA, siRNA, Protein (Native), snRNA, micro RNA
For Use With (Application)	Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Western Blotting, microRNA Analysis, Northern Blotting, cDNA Library Construction
High-throughput Compatibility	Not High-throughput Compatible (Manual)
No. of Reactions	40 Preps
Product Line	<i>mir</i>Vana, Ambion
Purification Time	30 min.
Quantity	40 preps
Shipping Condition	Room Temperature
Starting Material Amount	Up to 10^7 cells, Up to 100 mg tissue
Isolation Technology	Spin Column (Glass Fiber Filter)
Sample Type	Tissue (Mammalian)
Target	siRNA
Unit Size	Each

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